The inside-forward was the star man in a side which boasted several of his Benfica team-mates.
Benfica were one of the best club sides in Europe at the time.
They reached four of the five European Cup finals prior to the 1966 World Cup, and won two of them.
Eusebio possessed a powerful physique, blistering pace and his lethal right foot saw him take the Golden Boot with nine goals.
He almost single-handedly won the quarter-final at Goodison Park when Portugal were on the verge of an embarrassing defeat to North Korea.
They trailed 3-0, but four goals from Eusebio, including two from the penalty spot, sparked a dramatic comeback to secure a 5-3 victory.
By that stage many felt Portugal were good enough to win the tournament.
But with home advantage, and two goals from Bobby Charlton, England edged their semi-final meeting at Wembley.
Another late Eusebio penalty threatened a fightback but it was to be England's day.
Portugal went on to finish third when a Eusebio penalty and an effort from tall Benfica striker Torres won the play-off with Russia 2-1.
After that, Portugal vanished from the World Cup scene until 1986.
In Mexico, they achieved a modicum of revenge over England, beating them 1-0 in their first match.
But defeats to Poland and Morocco saw them finish bottom of the group.
They are almost certain to do much better this time around.