The All England Club has confirmed that Hawk-Eye technology will be used at Wimbledon this year for the first time.
The electronic system, which allows players to use video replays to question line calls, will be used on Centre Court and Court Number One.
Players will be allowed three incorrect challenges a set.
Hawk-Eye is already in use at the US and Australian Opens and makes its grass-court debut at the Artois Championships at Queen's Club in June.
World number one and four-time Wimbledon champion Roger Federer is not a fan of the system.
"Now the umpires can hide even more behind these calls, that's for sure," said Federer.
"It makes it really hard for us."
But All England Club chief executive Ian Ritchie said: "Statistics show that players have been very sensible in their use of the technology so far.
"It is right to grant them additional opportunities to use this highly accurate assistance."
If a set goes to a tiebreak, the limit of incorrect challenges is increased from three to four for the set, but challenges cannot be carried over between sets.
In the final set - fifth for men, third for women - the rules alter slightly, with an additional three incorrect challenges permissible if the match reaches 6-6.