Andre Agassi's 20-year career ended in a 5-7 7-6 (7-4) 4-6 5-7 defeat by German qualifier Benjamin Becker at the US Open on Sunday.
The 36-year-old was clearly affected by the chronic back injury which hastened his decision to retire.
Backed by a fiercely patriotic crowd, Agassi battled courageously but Becker's power proved too much.
The American legend fought back tears as the Arthur Ashe crowd, along with Becker, rose to applaud him.
Still crying, Agassi paid a sentimental tribute to his fans.
"The scoreboard shows that I lost today, but what it doesn't show is what I found," he said.
"Over the last 20 years I have found loyalty, you have pulled me through on court and also in life.
"He's one of a kind - he gave so much to tennis"
"I have found inspiration - you have willed me to succeed - and I have found generosity.
"I thank you and I will take you and the memory with me for the rest of my life."
Becker kept his nerve brilliantly in front of a crowd who were virtually silent whenever he won a point.
The German afterwards paid tribute to Agassi's contribution to the game.
"He's one of a kind," said the 25-year-old.
"He gave so much to tennis. He's such a gentleman and this is a great moment for him.
"Whatever they do for him he deserves it. I wish him all the best - I know he will be very happy in life."