Jason McIntyre built on his success in the national 25-mile championships to become British Time Trial Champion.
Jonathan Dayus shared second place with Michael Hutchinson ahead of Stuart Dangerfield over three laps of an 11-mile circuit in Hampshire.
In the women's race, Rebecca Romero beat team-mate Wendy Houvenaghel and previous champion Julia Shaw.
Britain's Olympic pursuit champion Bradley Wiggins opted not to make his debut in the event.
British Time Trial Championships results:
Men (3 laps)
1 J MacIntyre (Equipe Velo Ecosse) 1.11.15
2= J Dayus (Arctic Shorter Rochford) 1.11.48
2= M Hutchinson (In Gear Quickvit RT) 1.11.48
4 S Dangerfield (Science In Sport.com) 1.12.26
5 B Instone (A3crg) 1.13.15
Women (2 laps)
1 R Romero (SIS.com) 52.24
2 W Houvenaghel (SlS.com) 52.39
3 J Shaw (GS Strada) 53.56
Juniors (2 laps):
Male:
1 A Dowsett 49.08
2 R Meadows 49.42
3 A Griffiths 50.18
Female:
1 J Allen 57.03
2 J Rowsell 58.22
3 H Manley 1:06:12
Masters (2 laps):
Category A: M Wareham 49.37
B: S Walkling 48.28
C: I Dalton 49.22
D: K Tye 49.06
E: P Bull 50.42
F: R Robinson 50.53
G: R Iddles 51.17
H: M Ives 54.50
I: J Woodburn 53.22
Espoirs (2 laps)
1 D Davies 47.58
2 J Stewart 48.10
3 D Appleby 49.41