Port Talbot host Haverfordwest, Cwmbran welcome Carmarthen, Connah's Quay take on Aberystwyth and Caernarfon travel to Welshpool.
Holders Rhyl face a potential banana skin at Cymru Alliance side Llanfairpwll, while Bangor will be keen to avoid an upset at near-by Llanrug.
The ties are to be played on Saturday, 30 September.
Welsh Cup second round draw in full:
Barry Town v Afan Lido
Bodedern v Bethesda Athletic
Brickfield Rangers v Airbus UK
Bridgend Town v Garden Village
Brymbo v NEWI Cefn Druids
Buckley Town v Llanfyllin Town
Caerleon v UWIC
Connah's Quay Nomads v Aberystwyth Town
Cwmbran Celtic v Cardiff Corries
Cwmbran Town v Carmarthen Town
Denbigh Town v Newtown
Dinas Powys v Morriston Town
Ely Rangers v Caerau Ely
Goytre Utd v West End
Guilsfield v Bala Town
Llandudno v Flint Town
Llanelli v Bettws
Llanfairpwll v Rhyl
Llanegfni v Holyhead Hotspurs
Llanrug Utd v Bangor City
Mynydd Isa v Caersws
Neath Athletic v Llanwern
Newport YMCA v Ammanford
Penrhyncoch v Conwy United
Pontardawe Town v Pontypridd Town
Port Talbot Town v Haverfordwest County
Prestatyn v CPD Porthmadog
Presteigne St Andrews v Penycae
Pwllhelli v Gresford Athletic
The New Saints v Llanrwst United
Ton Pentre v Llangeinor
Welshpool Town v Caernarfon Town