Steve Finnan and Steven Reid will miss the Republic of Ireland's World Cup qualifier against Cyprus at Croke Park on 15 October.
The duo, who played in the qualifiers against Georgia and Montenegro last month, are ruled out by injury as is Birmingham defender Stephen Kelly.
Newcastle Damien Duff returns to the squad after missing the win over Georgia and the draw in Montenegro.
Goalkeeper Joe Murphy also earns a recall to the squad.
Finnan is ruled out after sustaining a calf strain in his debut for Spanish side Espanyol a couple of weeks ago while Kelly is unavailable because of a thigh injury.
Reid has missed Blackburn's last three games because of a knee injury and his absence is be a major blow for Giovanni Trapattoni.
The Republic boss has included Liam Miller in the squad even though that Sunderland midfielder has been troubled recently by a groin strain.
Despite his midfield problems which include the self-imposed absence of Stephen Ireland, Trapattoni has elected not to recall Lee Carsley and the Birmingham player's international days could now be over.
Paul McShane has been playing mainly at full-back since his loan move to Hull and he looks in line to replace Finnan.
Goalkeepers: S Given (Newcastle Utd), D Kiely (West Brom), J Murphy (Scunthorpe Utd)
Defenders: A Bruce (Ipswich Town), D Delaney (QPR), R Dunne (Manchester City), K Kilbane (Wigan Athletic), J O'Brien (Bolton), J O'Shea (Manchester Utd), P McShane (Sunderland - on loan at Hull City)
Midfielders: S Hunt (Reading), A McGeady (Celtic), D Duff (Newcastle Utd), L Miller (Sunderland), A Reid (Sunderland), G Whelan (Stoke City)
Forwards: K Doyle (Reading), R Keane (Liverpool), A Keogh (Wolves), S Long (Reading), D Murphy (Sunderland)
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