Amir Khan will face unbeaten Colombian Breidis Prescott at Manchester's MEN Arena on Saturday 6 September.
Khan, who stopped Michael Gomez in June, will look to extend his unbeaten run to 19 fights since turning pro.
The 21-year-old Bolton boxer will be defending his fringe intercontinental lightweight crown against an opponent who boasts 17 knockouts from 19 fights.
Khan will fight on the same bill as Alex Arthur's WBO super-featherweight defence against Dagenham's Nicky Cook.
Prescott's record reads well at first glance, with 14 of his fights ending inside three rounds, though his opponents have been of questionable quality and he is not ranked in the top 10 by any of the four major organisations.
However Khan, ranked number two with the WBO and five by the WBC, said the 25-year-old would provide a real test.
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"He may have been knocking them out left right and centre, but I'm going to make him wake up and smell the coffee.
"I've been working on my defensive skills with new coach Jorge Rubio, and it looks like Prescott will be testing out what I've been learning in the gym.
"Along with my strength and conditioning coach, I'm also punching harder than ever before, and I think my offensive skills are going to win me this fight."
Prescott, who has a height and reach advantage and has fought at light-welterweight before, predicts he can add another quick win to his record.
"Khan is just a boy. I will knock him out," he said.
Khan has also announced a 20-fight deal with Sky Box Office having ended his association with ITV.
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