British number one Anne Keothavong failed to reach the main draw at the Pacific Life Open in California after losing her final match in qualifying.
Keothavong has won 12 ITF circuit titles in her career
The 23-year-old, ranked 161, lost 7-6 (7-5) 6-3 to China's Meng Yuan, who is ranked one place above her, in Indian Wells on Tuesday.
The Londoner had beaten France's world number 115 Youlia Fedossova 6-3 2-6 6-3 on Monday.
Keothavong, who plays mainly on the ITF circuit, now has a 9-8 record for 2007.