Kim Clijsters made a winning return in her first match on grass for two years, beating Jelena Jankovic at the Hastings Direct International in Eastbourne.
But the Belgian was made to battle by the Serbian before wrapping up a 6-1 7-6 victory in windy conditions.
Elena Likhovtseva became the first big name to go out, the sixth seed losing 7-6 6-2 to France's Marion Bartoli.
Elena Baltacha, Britain's last remaining player, went out 7-5 2-6 6-1 to Spanish veteran Conchita Martinez.
The British number one put up a fight over two hours and 10 minutes on a wind-blown Centre Court.
"I played really well for the first two sets but suddenly I just lost it," said a frustrated Baltacha afterwards.
"I'm angry, of course, but I was just really poor at the start of the third set. Conchita is still a good player."
Clijsters came to Eastbourne a week early to get in as much practice as possible on grass.
And the 22-year-old is feeling relaxed as she prepares for Wimbledon.
"I have really enjoyed it," said Clijsters. "It's been fun. I could do my own thing and didn't have to worry about anyone else."
Katarina Srebotnik also went through after a slow start against Vera Douchevina, winning 1-6 6-2 6-2.
The top four seeds, Amelie Mauresmo, defending champion Svetlana Kuznetsova, Alicia Molik and Anastasia Myskina, all received byes.