World number two Kim Clijsters has pulled out of the French Open because of a persistent wrist injury.
Clijsters must rest for five weeks
Clijsters made an unsuccessful comeback after six weeks out with a torn wrist tendon this week when she was forced to withdraw from the German Open.
And she announced on her personal website that she would not be fit for Roland Garros, which starts on 24 May.
"The doctor has told her to rest. It is yet another setback," the Belgian's coach Marc Dehous said.
"It's not just the wrist that's the problem. There's also an accumulation of fluid around the tendons.
"There is no chance [of her playing in Paris]. Otherwise it may become a chronic injury.
"She will do everything possible to get healthy again as soon as possible."
Clijsters, who lost to compatriot Justine Henin-Hardenne in last year's French Open final, has one of the busiest schedules in tennis.
She played more than 100 singles matches last year, along with about 50 doubles matches.
Clijsters' wrist has been in a brace for more than a month and she has been told to rest it for at least another five weeks.
Before her injury, she had been in good form, winning back-to-back tournaments in
Paris and Antwerp.
Her world ranking is in no immediate danger as number three Amelie Mauresmo is well down in the WTA standings.
She will now focus on Wimbledon, which begins on 24 June.