Pete Sampras will officially announce his retirement at a ceremony at the US Open on Monday.
The 14-time Grand Slam winner has not played a competitive match since winning the title at Flushing Meadows last September.
Ever since June this year - when Sampras announced he would not be playing at Wimbledon - there has been mounting speculation that the 32-year-old would never play again.
"Not being at the Open this year and not playing Wimbledon hasn't made me miss tennis enough to
really start training, start practising, doing everything I have to do to be where I want to be," Sampras said last month.
"This tells me it's a sign to say, 'it's probably time'. I miss it, but it's time probably to give up.
"I feel like if I wasn't going to play Wimbledon, then that was probably it for me, so that's the way it goes."
Sampras's management, IMG, said Sampras would hold a news conference on Monday, followed by a special ceremony.
The US Open is the site of some of Sampras's greatest triumphs.
His first victory there in 1990 made him the youngest ever men's champion at 19 years and 28 days and his most recent triumph came when he was 31.
In addition to his five US Open singles titles, Sampras has won seven Wimbledon titles and two Australian Open crowns.