Robinho and Ronaldhino are both missing from Brazil's squad
AC Milan forward Robinho has been dropped from the Brazil squad to face Scotland in London on 27 March - and there is also no recall for Ronaldinho.
The Brazil captain appears to have paid the price for indiscipline that marred February's 1-0 friendly loss to France.
Lazio midfielder Hernanes is also absent after his red card in Paris.
But Inter Milan defenders Lucio and Maicon, along with Santos midfielder Elano, are called up for the first time since last year's World Cup finals.
South American football expert Tim Vickery was at Brazil coach Mano Menezes' media conference.
"Menezes had said players left out after the World Cup would be recalled at some point and that has come now," Vickery told BBC Scotland.
"With this game, his priorities change and he has gone with a slightly more experienced squad."
Menezes, whose side have lost their last two friendlies against Argentina and France, now has his eyes firmly on the Copa America in July.
That tournament, along with the Confederations Cup, provides Brazil's only competitive fixtures before they host the 2014 World Cup finals.
So friendlies like the one against the Scots at Emirates Stadium are of particular significance for Menezes.
However, the dropping of Robinho, who pulled the referee's shirt during the defeat by France, appears to be a signal that he will not tolerate the indiscipline that also marred their performance during the last finals in South Africa.
There had been speculation that former Barcelona forward Ronaldhino, now playing for Flamengo, would earn a recall.
However, the 30-year-old, who has played only once for Brazil in two years and was dropped from last year's World Cup squad, has again failed to make Menezes' squad.
Santos striker Neymar, though, is also called up after missing the previous match and midfielder Lucas, one of the team's stars at the South American under-20 tournament earlier this year, is included.
Three of the Brazil squad are based in London - Chelsea pair David Luiz and Ramires and Tottenham Hotspur midfielder Sandro - while Liverpool's Lucas Leiva is also on duty for the fixture.
Scotland are seeking their first win over Brazil after nine competitive and friendly fixtures. Head coach Craig Levein will announce his squad on Thursday 17 March.
Menezes dismissed suggestions that Wednesday's stormy derby between Rangers, who had three players sent off, and Celtic was a warning that his team would face an especially physical game against the Scots.
Meanwhile, world governing body Fifa on Thursday confirmed that Brazil would have an automatic berth at the 2014 finals and rejected North and Central American demands for four full places each.
The balance between the different continents among the 32 participants will be based on the same principle as in 2006 and 2010.
Africa will revert to its previous number after gaining one additional slot in the 2010 event because of host South Africa's automatic qualification, while Europe retains 13 places.
Goalkeepers: Victor (Gremio), Jefferson (Botafogo), Julio Cesar (Inter Milan)
Defenders: Lucio, Maicon (both Inter Milan), Thiago Silva (AC Milan), David Luiz (Chelsea), Luisao (Benfica), Marcelo (Real Madrid), Andre Santos (Fenerbahce), Daniel Alves (Barcelona)
Midfielders: Lucas (Sao Paulo), Lucas Leiva (Liverpool), Elano (Santos), Elias (Atletico Madrid), Henrique (Cruzeiro), Jadson (Shakhtar Donetsk), Ramires (Chelsea), Sandro (Tottenham Hotspur), Renato Augusto (Bayer Leverkusen)
Forwards: Alexandre Pato (AC Milan), Jonas (Valencia), Neymar (Santos), Nilmar (Villarreal)