Wales and France have played in the previous Six Nations Friday night games
Wales's game with England next week will be the last Friday night match in the Six Nations for the foreseeable future.
Championship chief executive John Feehan has confirmed the experiment will be scrapped for 2012 and 2013.
The Six Nations will still end with three games on Saturday but on every other weekend there will be two games on the Saturday and one on the Sunday.
The introduction of Friday night Six Nations games has proved controversial.
Wales play England at the Millennium Stadium on 4 February in the first Friday night opener of a Six Nations Championship in its 128-year history.
With stadia at full capacity, television is the best way to increase the number of people watching matches. We feel that the fixture schedules for the next two years will deliver on this
Six Nations chief executive John Feehan
The match is also the first time that two home union sides will meet on a Friday night as France and Wales have competed in the previous fixtures.
France met Wales at the Stade de France in 2009 in the first Friday night game in the Championship's history - a 21-16 home victory - before the corresponding fixture in Cardiff in 2010, which France won 26-20.
The Six Nations' Friday night trial has not been popular with supporters or players but the matches enjoyed bumper television viewing figures.
Rail companies warned of potential disruption and some train services into Cardiff look set to be disrupted when England visit Wales as rail union RMT has confirmed it will strike on 4 February after talks with Arriva Trains Wales over pay broke down.
Despite the end of the Friday night experiment, Feehan is confident it will not affect TV audiences.
"With stadia at full capacity, television is the best way to increase the number of people watching matches," he said.
"We feel that the fixture schedules for the next two years will deliver on this."
Six Nations fixtures for 2012 and 2013:
France v Italy (1430 GMT)
Scotland v England (1700 GMT)
Ireland v Wales (1500 GMT)
Italy v England (1600 GMT)
France v Ireland (2000 GMT)
Wales v Scotland (1500 GMT)
Ireland v Italy (1330 GMT)
England v Wales (1600 GMT)
Scotland v France (1500 GMT)
Wales v Italy (1430 GMT)
Ireland v Scotland (1700 GMT)
France v England (1500 GMT)
Italy v Scotland (1230 GMT)
Wales v France (1445 GMT)
England v Ireland (1700 GMT)
Wales v Ireland (1330 GMT)
England v Scotland (1600 GMT)
Italy v France (1500 GMT)
Scotland v Italy (1430 GMT)
France v Wales (1700 GMT)
Ireland v England (1500 GMT)
Italy v Wales (1430 GMT), England v France (1700 GMT)
Scotland v Ireland (1400 GMT)
Scotland v Wales (1430 GMT), Ireland v France (1700 GMT)
England v Italy (1500 GMT)
Italy v Ireland (1430 GMT), Wales v England (1700 GMT), France v Scotland (2000 GMT)