The Welsh Rugby Union is facing the threat of an Extraordinary General Meeting for the second time in a week.
Llangefni RFC are contesting the WRU's decision to deny them promotion, and have collected the required number of signatures to call for an EGM.
The Anglesey club have been told they must drop a division next season to play in the new Division Four North.
The WRU says the changes are part of a new national structure to re-organise leagues on a regional basis.
The changes are also designed to help cut travelling costs for the clubs, according to the WRU.
But Llangefni, who finished second in Division Three West last season, say they are more than happy to travel to south Wales every fortnight to play in Division Two West.
"We have contacted all the north Wales clubs and every one of them is in full support of what we are trying to do," said Llangefni secretary Gareth Luke.
"We've been trying to get the WRU north Wales council to support us since last December but they have failed to do so.
"So the time has come that we are going to call an EGM because we must get amendments to the so-called strategic document for north Wales.
"I'd like to know what is the WRU's thinking. It's clear to us that the decision to keep north Wales [clubs] separate from the south is discrimination.
"We're a small enough country as it is. How they can treat us from any other part of Wales I don't know?"
Beddau and Bonymaen are also threatening to call an EGM after they were denied promotion to the Premiership after failing ground criteria.
The Division One clubs are meeting the WRU board on Friday in an attempt to have the decision reversed.