Barkley kicked five points for Bath at Gloucester before his injury
Bath centre Olly Barkley will miss the rest of the season after breaking his left leg in two places in Saturday's 34-22 defeat at Gloucester.
The 29-year-old fractured his lower tibia and fibula and underwent an operation hours after leaving the pitch wearing an oxygen mask.
A club statement said doctors were pleased with how the surgery had gone.
Director of rugby Ian McGeechan said: "It is very disappointing for Olly to suffer an injury of this nature."
He added: "He had been playing so well recently. It is doubly disappointing for Olly and the club as he also missed much of last season with a bad leg injury.
"We will of course make sure he receives the best possible medical attention to get him back on the field as and when he is ready."
Barkley, who has won 23 England caps, also confirmed the injury via his
It's a cruel blow especially at this end of the season
Bath chief executive Nick Blofeld
He said: "For those that have asked I clean broke my tibia and fubula and had a rod with a few screws put through my tib to secure it. Fib will heal."
It was Barkley's first match at Kingsholm since his one-season stint with the club in 2008/09 and it is the same leg he broke prior to the start of the subsequent campaign after returning to Bath.
He suffered the injury in a collision with team-mate Butch James as Bath pushed for a try while trailing 26-15.
Bath chief executive Nick Blofeld told BBC Radio Bristol: "He's going to be out for a few months, unfortunately.
"It's a cruel blow especially at this end of the season.
"Gloucester have been brilliant and the good thing about being at Kingsholm is that it's just around the corner from the hospital so hopefully it's gone very smoothly and he'll be back on his feet as soon as possible.
"We've definitely had better days. Olly is a key member of the team so it was a frustrating day."