Ireland A enjoyed a well-earned victory at Kingsholm on St Patrick's night.
Jeremy Staunton scored one of Ireland's tries
The Irish led 18-11 at half-time with tries by Ian Dowling and Jeremy Staunton, with Staunton adding two penalties and a conversion.
Kieran Lewis and Rob Kearney added second-half touchdowns and Staunton a further penalty and conversion.
England's tries were scored by Paul Sackey and Michael Lipman, with fly-half Shane Drahm contributing two penalties and a conversion.
England A: M Van Gisbergen, P Sackey, J Clarke, O Smith, C Bell; S Drahm, S Perry (capt); T Payne, A Titterrell, S Turner, L Deacon, T Palmer, A Beattie, M Lipman, C Jones.
Replacements: M Cairns, N Wood, D Ward-Smith, T Rees, N Walshe, S Vesty, D Armitage
Ireland A: R Kearney; T Bowe, G Duffy, K Lewis, I Dowling; J Staunton, T O'Leary; R Hogan, J Fogarty, B Young, M O'Driscoll (capt), M McCullough, N Best, S Jennings, J Heaslip.
Replacements: B Blaney, R McCormack, L Cullen, R Wilson, C Keane, P Wallace, B Cunningham.