Italy prop Martin Castrogiovanni has been ruled out for the rest of Six Nations after suffering a knee injury against Scotland last weekend.
Castrogiovanni will miss some key games for Leicester
The 25-year-old Leicester forward sprained his right knee in the Murrayfield win and is expected to be sidelined for up to six weeks.
Castrogiovanni joins Andrea Masi and Fabio Ongaro on the sidelines for the home game with Wales on 10 March.
Fabio Staibano, Leonardo Ghiraldini and Ezio Galon have been named as cover.
Staibano takes Castrogiovanni's place while Ghiraldini replaces Ongaro.
Wing Galon replaces centre Masi, who suffered a facial injury against Scotland.
Backs: Mirco Bergamasco, Gonzalo Canale, Roland De Marigny, Ezio Galon, Paul Griffen, Ramiro Pez, Matteo Pratichetti, Kaine Robertson, Andrea Scanavacca, Alessandro Troncon.
Forwards: Mauro Bergamasco, Valerio Bernabo, Marco Bortolami (c), Santiago Dellape, Carlo Festuccia, Leonardo Ghiraldini, Andrea Lo Cicero, Roberto Mandelli, Carlos Nieto, Sergio Parisse, Salvatore Perugini, Fabio Staibano, Maurizio Zaffiri, Alessandro Zanni.