France fly-half Frederic Michalak will miss the Six Nations after rupturing an interior ligament in his right knee.
Michalak was injured during Toulouse's win against Bourgoin
Michalak, who had only just returned from two months out, suffered the injury in Toulouse's domestic league win over Bourgoin last Friday.
The player will now undergo surgery on Friday, which will leave him sidelined for at least four months.
Meanwhile, France captain Fabien Pelous will miss Saturday's Test against New Zealand with a rib injury.
The 32-year-old lock, who would have earned a record-equalling 111th French cap in the Stade de France clash, picked up the injury in Saturday's 47-3 defeat to the All Blacks.
Pelous will be replaced in the 22-man squad by uncapped Clermont Auvergne player Loic Jacquet, who is likely to be on the bench with Lionel Nallet promoted to the starting line-up.
Wasps hooker Raphael Ibanez is set to take over the captain's armband.