Brewery Field, Bridgend
Sunday, 16 October
Kick-off: 1500 BST
Warrington Wolves stand-off Lee Briers captains a Wales squad that includes 10 players with Super League experience.
Briers won his last cap against New Zealand in Cardiff in 2002
Their only injury worries are Widnes pair Adam Hughes and Gary Hulse, who will be given until the morning of the match to prove their fitness.
Scotland are set to hand debuts to Wade Liddell, Jon Steel, Ben Fisher, Dougie Flockhart and Nick Surtees.
Uncapped forwards Dave Simm and Steve Deviney also vying for a bench spot for the game at Bridgend's Brewery Field.
Hull KR's Steel, a former union international, will become the sixth Scotland player to be capped at both league and union since the barriers came down in 1995.
Scottish preparations have been hit by the loss of skipper Wayne McDonald and Duncan MacGillivray.
Huddersfield's McDonald stepped down for personal reasons while Wakefield Trinity second rower MacGillivray needs surgery on a groin problem.
Wales half-back Mark Lennon is confident his side can get their European Nations Cup campaign off to a winning start.
"We've got the right combination of youth and experience and I believe we have the necessary talent to win this tournament," said the Manly Sea Eagles player.
"Lee Briers' influence has been tremendous. He's been captain of Warrington for a few years now, so you can see how good his leadership skills are.
"As well as all the Super League players, we've also got a great bunch of lads from the Welsh Conference training with us.
"All of them are showing great promise and should bode well for Welsh rugby league if they are picked for the Celtic Crusaders next year."
Wales squad: L Briers (Warrington, capt), D Gibson (Halifax), A Blackwood (Halifax), Nick Royle (Rochdale), M Lennon (Manly), B Powell (Featherstone), A James (Sheffield), B Smith (Castleford), G Dean (Carcassonne), D Mills (Widnes), G Price (Rochdale), I Watson (Swinton), J James (Castleford), P Joseph (Swinton), C Morley (Halifax), H O'Hare (Glasgow), A Hughes (Widnes), G Hulse (Widnes), K Mason (St Helens), J Breakingbury (Valley Cougars), D Hudd (Torfaen Tigers), N Strong (Bridgend Blue Bulls), K Hocking (Bridgend Blue Bulls), A Llewellyn (Aberavon Fighting Irish), N Davies (Aberavon Fighting Irish), S O'Kelly (Aberavon Fighting Irish), L Williams (Aberavon Fighting Irish), M Burke (Aberavon Fighting Irish).
Scotland squad: Danny Brough (Hull FC), Andy Brown (Fife Lions), Steve Deviney (Easterhouse Panthers), Ben Fisher (Halifax), Richard Fletcher (Castleford Tigers), Dougie Flockhart (Scotland Students), Andrew Henderson (Castleford Tigers), Wade Liddell (Gateshead Thunder), David McConnell (Rochdale Hornets), Andy McPhail (Clyde Bulls), Iain Marsh (Batley Bulldogs), Gareth Morton (Hull KR), Mick Nanyn (Whitehaven), David Simm (Edinburgh Eagles), Ian Sinfield (Swinton Lions), Jon Steel (Hull KR), Nick Surtees (St Albans Centurions), Alex Szostak (Bradford Bulls), Mike Wainwright (Warrington Wolves), Oliver Wilkes (Leigh Centurions).