Castleford's Danny Orr has been named as Yorkshire captain for this year's Origin game.
Andy Farrell is also captain of Wigan, England and Great Britain
Wigan's Andy Farrell will captain reigning champions Lancashire when the two sides meet at Odsal on 2 July.
Farrell, who is also skipper of his club and Great Britain, has led the Red Rose county to victory in all three clashes since they were revived in 2001.
Orr, who has led the Tigers for the last two seasons, pipped Bradford duo Stuart Fielden and Jamie Peacock to the post.
"These are the games you want to play in," said Orr, who broke into the Lions team in last year's Test series against New Zealand.
This is a real chance for guys to step up above
the level of Super League and show what they can do in an intense match against
Lancashire coach Paul Cullen
"You are up against the cream of the crop and it's very exciting."
Lancashire coach Paul Cullen, boss of Super League side Warrington, said his choice of captain was one of the easiest decisions he will have to make.
"Andy is a living legend of rugby league and the moment he declared himself fit, he was always going to be the captain of Lancashire," said Cullen.
Yorkshire boss Graham Steadman said he has already included "one or two surprise selections" in a provisional 22-man squad, which will be finalised by 24 June.
The Origin game is seen as a genuine Test trial for the November Ashes Series against Australia.