Tottenham's hopes of moving to London's 2012 Olympic Stadium have been dashed after Games organisers said athletics would remain its core use.
Spurs chairman Daniel Levy had hinted the club might move to the proposed 80,000-seater stadium in Stratford.
But London 2012's Jackie Brock-Doyle told BBC Sport this would not happen.
"We are committed to building a stadium with athletics at its core. The aim is to provide a sustainable post-Games legacy for athletics," she said.
The stadium will hold just 25,000 people after the Games, when the other 55,000 temporary seats are ripped out.
Tottenham are looking to expand their 36,000 capacity or move from their White Hart Lane home.
Fellow Premiership side Manchester City took over the City of Manchester Stadium following the 2002 Commonwealth Games.
Meanwhile, Brock-Doyle dismissed reports suggesting that it would cost more than the £250m set aside to build the stadium.
The new Wembley Stadium will cost the Football Association £757m and Arsenal's Emirates Stadium is expected to cost £365m.
But Brock-Doyle says such comparisons are unfair as Wembley will continue to seat 90,000 spectators.
"Essentially, the Olympic Stadium will be a fraction of the size of Wembley, and hence a fraction of the cost - you cannot compare a 90,000-seat stadium with a 25,000-seat one," she said.