Tony McCoy rides Albertas Run to victory in Cheltenham's Ryanair Chase
Cheltenham winner Albertas Run is among 23 horses to be withdrawn from the Grand National at Aintree on 10 April.
Albertas Run, who won the Ryanair Chase last week, joins the likes of War Of Attrition, Taranis and Possol in being scratched as 75 remained in the race.
Last year's Grand National winner Mon Mome, who finished third in the Cheltenham Gold Cup, will carry 11st 7lb - an increase of 7lb on last year.
Madison du Berlais and Notre Pere head the weights with 11st 10lb.
Although out of the National, Albertas Run could still run at the Aintree meeting - either in the Totesport Bowl on 8 April or the Melling Chase the day after.
Mon Mome, a surprise 100-1 winner of the National last year, is set to carry extra weight next month but trainer Venetia Williams says she will not be too concerned if that is the case.
"It's not the be all and end all," said Williams. "A horse can fall, you are always afraid of getting hampered, you never know what might happen.
"His preparation has gone a lot better than it did last year and we have got a blueprint to follow.
"We were all very buoyed by what we saw him do at Cheltenham. It might have been a bit cheeky running in the Gold Cup as a prep race for the National, but there was £50,000 for third place and we were delighted with what he did.
"The hill at Cheltenham compensated for the extra mile he will get in the National."
Only 40 horses will run in the event, with those on a weight of 10st 1lb or less not guaranteed to make the final field.
Champion trainer Paul Nicholls is chasing a first National win and has Big Fella Thanks and Tricky Trickster as possible contenders.
Williams has expressed her hope that Flintoff is able to get in. The horse, currently 49th on the list, is part owned by England cricketer Andrew Flintoff.
"Flintoff has had a problem with his foot, but he's in good form and is doing his work well. I hope he gets in," she added.
"There's no time for a prep run before the National for him now, but he was second in the Midlands Grand National last year first time out so we wouldn't be worried about going to Aintree fresh."
Silver Birch, who won the 2007 National as a 33-1 shot with Robbie Power on board, needs 11 horses to come out of the race to be guaranteed a run.
The 2008 winner Comply Or Die, who finished second last year, is certain of a place in the 2010 line-up.
The other withdrawals from the latest entry stage are: Taranis, Our Vic, Casey Jones, Roulez Cool, Le Beau Bai, Seven Is My Number, Louping D'Ainay, Gone To Lunch, Kornati Kid, Follow The Plan, Cane Brake, New Alco, Parsons Pistol, Iris De Balme, Trust Fund, Coe, Mumbles Head, Sizing Australia, Trabolgan and Boychuk.
The National is the only race in which weights are decided at the discretion of the British Horseracing Authority's Head of Handicapping, Phil Smith.
This year's spectacle over the four-mile, four-furlong course at Aintree will have a record £925,000 prize fund.
John Smith's Grand National entries Saturday 10 April, 1615 BST
A maximum field of 40 will be declared on Thursday
1 101-3244 MADISON DU BERLAIS (FR) 9-11-10 David Pipe
2 12-1F544U NOTRE PERE (FR) 9-11-10 Jim Dreaper IRE
3 001-P3463 MON MOME (FR) 10-11-07 Venetia Williams
4 P151U-02 BLACK APALACHI (IRE) 11-11-06 Dessie Hughes IRE
5 15-50030 JOE LIVELY (IRE) 11-11-06 Colin Tizzard
6 0-211501 VIC VENTURI (IRE) 10-11-06 Dessie Hughes IRE
7 1/P002-00 COMPLY OR DIE (IRE) 11-11-05 David Pipe
8 201-423P DON'T PUSH IT (IRE) 10-11-05 Jonjo O'Neill
9 3-3U2404P MADE IN TAIPAN (IRE) 8-11-04 Tom Mullins IRE
10 401-03520 NICHE MARKET (IRE) 9-11-04 Bob Buckler
11 22121-210 TRICKY TRICKSTER (IRE) 7-11-04 Paul Nicholls
12 31FU-3644 CLOUDY LANE 10-11-03 Donald McCain Jnr
13 PUPP/-21P DREAM ALLIANCE 9-11-03 Philip Hobbs
14 1P00-60 NOZIC (FR) 9-11-03 Paul Nicholls
15 553-2057 MY WILL (FR) 10-11-02 Paul Nicholls
16 3606-4F PABLO DU CHARMIL (FR) 9-11-02 David Pipe
17 0112313 BACKSTAGE (FR) 8-11-00 Gordon Elliott IRE
18 P6-51110 BALLYHOLLAND (IRE) 9-11-00 Colin McBratney IRE
19 FP-1P1PP BEAT THE BOYS (IRE) 9-11-00 Nigel Twiston-Davies
20 100-000U0 PREISTS LEAP (IRE) 10-11-00 Tom O'Leary IRE
21 314F-016 CAN'T BUY TIME (IRE) 8-10-13 Jonjo O'Neill
22 1103223 SNOWY MORNING (IRE) 10-10-13 Willie Mullins IRE
23 2506-P3UF AIR FORCE ONE (GER) 8-10-12 Charlie Mann
24 2U136-2U1 BIG FELLA THANKS 8-10-12 Paul Nicholls
25 113-41230 DEUTSCHLAND (USA) 7-10-12 Willie Mullins IRE
26 143301-00 CHARACTER BUILDING (IRE) 10-10-11 John Quinn
27 F0-52600 CHELSEA HARBOUR (IRE) 10-10-11 Tom Mullins IRE
28 02U-301F2 DOONEYS GATE (IRE) 9-10-11 Willie Mullins IRE
29 60/144-P STATE OF PLAY 10-10-11 Evan Williams
30 00-12100 ELLERSLIE GEORGE (IRE) 10-10-10 Nick Mitchell
31 2P-4P1540 BALLYFITZ 10-10-09 Nigel Twiston-Davies
32 043545U CONNA CASTLE (IRE) 11-10-09 Jimmy Mangan IRE
33 P23-15P6 EQUUS MAXIMUS (IRE) 10-10-09 Willie Mullins IRE
34 02P5-011 ERIC'S CHARM (FR) 12-10-09 Oliver Sherwood
35 212/0-0000 KING JOHNS CASTLE (IRE) 11-10-09 Arthur Moore IRE
36 F2-305467 OLLIE MAGERN 12-10-09 Nigel Twiston-Davies
37 330-30U2 ARBOR SUPREME (IRE) 8-10-08 Willie Mullins IRE
38 36244/-660 LENNON (IRE) 10-10-08 Howard Johnson
39 02P-253 MALJIMAR (IRE) 10-10-08 Nick Williams
40 U01-4U5 IRISH RAPTOR (IRE) 11-10-07 Nigel Twiston-Davies
41 6P/6220-0 MR POINTMENT (IRE) 11-10-07 Paul Murphy
42 000-33010 PIRAYA (FR) 7-10-07 David Pipe
43 3-012401P RAZOR ROYALE (IRE) 8-10-07 Nigel Twiston-Davies
44 03-02312 THE PACKAGE 7-10-07 David Pipe
45 11-035U00 HELLO BUD (IRE) 12-10-06 Nigel Twiston-Davies
46 1P6-1420 PALYPSO DE CREEK (FR) 7-10-06 Charlie Longsdon
47 23/32-31P ABBEYBRANEY (IRE) 9-10-05 Howard Johnson
48 50PP/05-0 CERIUM (FR) 9-10-05 Paul Murphy
49 P63/52-P FLINTOFF (USA) 9-10-05 Venetia Williams
50 56/-33P5 ROYAL ROSA (FR) 11-10-05 Howard Johnson
51 0F-26r20 SILVER BIRCH (IRE) 13-10-05 Gordon Elliott IRE
52 -3PP462F KNOWHERE (IRE) 12-10-04 Nigel Twiston-Davies
53 3025-0560 OODACHEE 11-10-04 Charlie Swan IRE
54 P4F1311 WHINSTONE BOY (IRE) 9-10-04 Jimmy Mangan IRE
55 0P03-402 FAASEL (IRE) 9-10-03 David Pipe
56 P/1r40P-0 OFFICIER DE RESERVE (FR) 8-10-03 Venetia Williams
57 115-30011 CHIEF DAN GEORGE (IRE) 10-10-02 Jimmy Moffatt
58 111P20 DUERS (IRE) 8-10-02 Paul Magnier IRE
59 P01-1F01 BALLYTRIM (IRE) 9-10-01 Willie Mullins IRE
60 20-0U004 OFFSHORE ACCOUNT (IRE) 10-10-01 Charlie Swan IRE
61 0/-P220P6 PAK JACK (FR) 10-10-01 Richard Phillips
62 366//4-547 KINGS ADVOCATE (IRE) 10-10-00 Tom Taaffe IRE
63 1/3F-116P TREACLE (IRE) 9-10-00 Tom Taaffe IRE
64 145-2613 GALANT NUIT (FR) 6-9-13 Ferdy Murphy
65 00F4215 ANOTHERCOPPERCOAST (IRE) 10-9-12 Paul Roche IRE
66 31F4-22 BELON GALE (IRE) 7-9-12 Howard Johnson
67 45-0600P POMME TIEPY (FR) 7-9-12 Willie Mullins IRE
68 1021-02D6 MERIGO (FR) 9-9-10 Andrew Parker
69 53414-00 CHIARO (FR) 8-9-09 Philip Hobbs
70 5/44-U335P ACCORDING TO JOHN (IRE) 10-9-08 Nicky Richards
71 24-512P0P LORUM LEADER (IRE) 9-9-07 Dr Richard Newland
72 1342/-0PP0 OVER THE CREEK 11-9-04 David Pipe
73 253-346 COSSACK DANCER (IRE) 12-9-01 Mark Bradstock
74 0/13P/-FP52 WEE ROBBIE 10-9-00 Nick Gifford
75 2U1/P6P-4P OFFALY (IRE) 9-8-12 Pru Townsley