Motivator won last year's Racing Post Trophy at Doncaster
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Motivator is to bypass the 2000 Guineas to begin his career as a three-year-old in the Dante Stakes at York on 12 May.
The unbeaten colt impressed on the Newmarket gallops on Tuesday alongside recent Doncaster winner Cool Panic.
But trainer Michael Bell and owners the Royal Ascot Racing Club have decided it is in his best interests to miss the first Classic of the season.
Motivator was as short as 10-1 for the Guineas. The Derby remains an option subject to a successful trial outing.
Royal Ascot Racing Club manager Harry Herbert said: "His physique and pedigree suggest he is a middle-distance horse, although he also possesses an abundance of speed.
"These decisions are never easy - there are so many pieces of the puzzle to fit together.
"We have sat down and decided that he will not now go for the 2000 Guineas and instead be aimed at the Dante."