The Jockey Club has charged four people over the running of Hillside Girl last June as the bad publicity surrounding horse racing continued.
Trainer Alan Berry, rider Paul Bradley, Steve O'Sullivan and Dale Jewett have all been implicated in running the horse despite knowing it was lame.
Farrier O'Sullivan is also alleged to have laid Hillside Girl to lose with betting exchange Betfair.
The filly had drifted from odds-on favourite to 22-1 outsider.
The charges come after a nine-month investigation following the Father's Day Novice Auction Stakes at Carlisle last June.
Berry's horse was pulled up lame after two furlongs, but the trainer denies any wrongdoing regarding the incident and plans to clear his name.
Berry and Bradley have also been charged with providing inaccurate information and omitting relevant information to the stewards after the initial investigation on the day of the race.
Jockey Club public relations director John Maxse said: "The race in question preceded by one day the implementation of a Memorandum of Understanding with betting exchanges which, under certain conditions, provides the Jockey Club with access to details of account holders.
"The progress of the investigation was also delayed by Steve O'Sullivan's reluctance to supply the Jockey Club with information relating to his betting accounts and telephone records, which were thought to be relevant to the security department's inquiry into the running of Hillside Girl.
"As a former registered stable employee of Alan Berry, O'Sullivan was previously bound by the Rules of Racing. Under Rule 241 any person subject to the Rules is in breach if they refuse to supply information requested by the Jockey Club.
"Steve O'Sullivan has previously disputed the relevance of all the information that was being requested."