By Elizabeth Hudson
BBC Sport at Wimbledon
Briton Jayant Mistry and France's Michael Jeremiasz's dream of back-to-back Wimbledon wheelchair doubles fell in Sunday's final.
Saida and Kunieda celebrate their victory
Mistry and Jeremiasz lost 7-5 6-2 to Japan's Paralympic champions Shingo Kunieda and Satoshi Saida.
The Anglo-French pair were well in contention in the opening set but Mistry was broken in the 11th game.
The Japanese duo never looked back after that, taking a 4-0 lead in the second set and wrapping up the victory.
Mistry and Jeremiasz, who beat Dutchman Robin Ammerlaan and Austrian Martin Legner in impressive style in Saturday's semi-finals, began in similar fashion again.
After the first four games were shared, Jeremiasz held serve to love with some superb serving and the pressure seemed to get to Saida, who struggled and was broken to love to leave Mistry and Jeremiasz 4-2 up.
But Mistry lost his next two service games, putting Kunieda and Saida 6-5 ahead and Kunieda safely held his serve to win the set.
The defending champions struggled at the start of the second set, both losing their serve in quick succession, and they found it difficult to cope with their opponents, who beat them in last week's Paris Open final.
Although Jeremiasz held to make it 4-1 and give them some hope, the damage had been done and once again Kunieda held his nerve to claim the famous victory.
"We are disappointed to lose this because we started well and were well in the match in the first set," said Mistry.
"But they came back strongly and we couldn't get enough balls in court, especially at the end of the first set and in the second set we let them get too far ahead of us."
Dutchman Robin Ammerlaan and Austrian Martin Legner beat Miroslav Brychta of the Czech Republic and Poland's Tadeusz Kruszelnicki 6-3 6-1 in the play-off for third and fourth place.