Lance Armstrong's US Postal team won the team time trial on stage four of the Tour de France, clocking a time of one hour 18 minutes and 27 seconds.
The American team finished 30 seconds ahead of last year's winners ONCE, with Jan Ullrich's Team Bianchi a further 13 seconds adrift in third.
The victory meant US Postal's Victor Hugo Pena took the overall lead and became the first Colombian ever to wear the yellow jersey in the race's 100-year history.
And reigning champion Lance Armstrong moved into second place just one second behind.
ONCE had looked set to repeat their 2002 success until US Postal picked up the pace over the second half of the 69-kilometre stage to easily secure their first stage win of this year's race.
I'll be the one wearing the yellow jersey, but this is a victory for the team
Their dominance was such that US Postal riders now occupy the top eight positions in the overall standings.
ONCE's Joseba Beloki in ninth is the leading rider from another team in the general classification.
Ullrich's challenge for the yellow jersey may have been hampered by the loss of two Team Bianchi riders during the stage as they failed to match US Postal's increase in pace in the later stages.
It was also a disappointing afternoon for Britain's David Millar as his Cofidis team failed to shine and finished over two minutes behind the winners.
New race leader Pena paid tribute to his team's efforts: "Sure, I'll be the one wearing the yellow jersey, but this is a victory for the team - it's one of the best teams
that's ever existed in the world."
"This is simply the proof that when he wants to be, Armstrong can also be the best team-mate in the world."
Full results to follow.