Johan Bruyneel will be investigated by the Royal Belgian Cycling League
Lance Armstrong's team manager is to be investigated over drugs-related allegations made by Floyd Landis.
RadioShack's Johan Bruyneel is one of four people into whom inquries will be made by their national federation under orders from the sport's governing body.
Garmin-Transitions sports director Matthew White, Team Sky rider Michael Barry and BMC manager John Lelangue will also come under the spotlight.
Armstrong has issued a statement saying Landis has "zero credibility".
The International Cycling Union (UCI) said: "The UCI has...requested the relevant national federations to carry out inquiries into the accusations made by Mr. Floyd Landis against their licence-holders.
"Namely Cycling Australia (Matthew White), Royal Belgian Cycling League (Johan Bruyneel), Canadian Cycling Association (Michael Barry) and the French Cycling Federation (John Lelangue).
"The UCI's request is aimed at establishing, in an objective manner, whether or not events potentially constituting a breach of the anti-doping rules occurred."
The UCI also stated that allegations made by Landis against former US Postal team-mate and seven-times Tour de France champion Armstrong were already being investigated.
"An inquiry has also already been opened by the United States Anti-Doping Agency (USADA) concerning all the other individuals accused by Mr. Landis as these persons have U.S. nationality (Lance Armstrong, George Hincapie, Levi Leipheimer, Jim Ochowicz and David Zabriskie)," it added.
Landis, who was stripped of his 2006 Tour de France title after a positive test for testosterone, admitted to doping last week and accused Armstrong and others of also cheating.
Armstrong has often been accused by his rivals and critics of cheating but has never officially failed a doping test and has always maintained his innocence.
A statement by Team RadioShack's lawyers said: "Lance looks forward to once again being totally vindicated after a fair investigation."