Queally will be in action almost 10 years after winning gold in Sydney
Former Olympic kilometre time trial champion Jason Queally has been named in the Great Britain squad for this month's World Championships in Denmark.
The 39-year-old retired after failing to make the sprint squad for the 2008 Games but has now targeted the 2012 London Olympics when he will be 42.
Queally had been riding as a tandem pilot with the British Paralympic team.
However, his selection for Copenhagen leaves partner Anthony Kappes looking for a new pilot ahead of 2012.
Sir Chris Hoy and defending women's sprint champion Victoria Pendleton are among the leading figures set to compete in world cycling's big event in Copenhagen from 24-28 March.
Following his decision to quit able-bodied competition, Queally switched to the Paralympic team but had to sit out a two-year eligibility period from the date of his last able-bodied event before being due to race next month.
It will be tough, but London is a once in a lifetime opportunity
In a special demonstration at last year's Paralympic World Cup in Manchester, he and Kappes set a new British tandem record and were training towards the London 2012 Paralympics.
But after working on his pursuit skills for the Paralympic events, he earned a call-up into the Olympic team pursuit training squad.
"At 40, I was never going to get an opportunity like this again," said Queally.
"Even if I don't make it to London, I know I have had the opportunity to go for it. If I'm successful, fantastic, if not, I have given it my best shot."
GB performance manager Shane Sutton said: "The worlds in Copenhagen will be about assessing where we are, right here, right now.
"We know what is going to be needed at the London Olympics so we need to go to the worlds and assess where we are and how far we have got to go.
"I've noticed a high morale across the team and there is a bit of buzz around these worlds as the days to 2012 are counting down."
The Paralympic squad already have two other pilots in former Olympian Craig Maclean, who will be eligible to compete from the start of November, and Barney Storey, who partnered Kappes to double gold in Beijing.
"Anthony is still our number one stoker and he won't be without a ride," team performance director Gareth Sheppard told the BBC.
"We have always rotated our pairings in the past and will continue to do that.
"It's always disappointing to lose a top-class athlete like Jason from our programme but we are lucky to have strength in depth.
"With the postponement of the World Para-cycling Track Championships until November or December, it could work in our favour with Craig now potentially being eligible to race."