She leads a seven-woman team, including Pooley and rising star Lizzie Armitstead, in Saturday's event.
"To be truthful I'd say I'm not in the same condition I was last year," Cooke told BBC Radio Wales.
"I had an illness in July that has taken a lot more to recover from than I expected, so I've done my best to try and be at my best.
"I'm really looking forward to it. I've been training on the course in the last week, getting to know the climbs, the descents and how to pace myself.
"It's very tough. We've got two climbs each lap and nine laps to do in three hours. It will make for a very exciting race."
Great Britain will also have high expectations for Peter Kennaugh, who will race for GB-based professional squad Team Sky next season and takes part in Saturday's Under-23 men's road race.
Earlier on Wednesday, Australian Jack Bobridge - who will ride for the Garmin professional team next year - won the men's Under-23 time trial, with Britain's Alex Dowsett seventh, 43.96 seconds behind the winner.
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