Pooley also won her second Road World Cup event earlier this month
Emma Pooley emulated Great Britain team-mate Nicole Cooke with victory in the Grande Boucle Feminine - the women's Tour de France.
Pooley, who won Olympic time-trial silver in Beijing, clinched overall victory by 22 seconds.
The Londoner, 27, won the individual time-trial on day one and triumphed in stage three by 20 seconds on Saturday.
She consolidated her lead with a fifth-place finish in Sunday's final stage to follow up Cooke's 2006 and 2007 wins.
Pooley's Cervelo Test Team team-mate Christiane Soeder of Austria was second, with Marianne Vos of Holland third in the four-stage race.
Welsh star Cooke (Vision 1 Racing), who is the world and Olympic road race champion, won the Giro del Trentino in Italy last Friday.