This year's Tour of Britain will end in London and has been expanded to six days to include a stage in Scotland.
The event, successfully re-launched last year after a five-year absence and won by Mauricio Ardila of Colombia, will start in Glasgow on 30 August.
It then moves on to stages in Cumbria and Lancashire, Yorkshire, the East Midlands, Birmingham and London.
The final stage in London on 4 September will conclude with a circuit race around Westminster.
The event will be preceded by a similar circuit race around Glasgow's George Square.
And the opening stage will see the 96 cyclists head south from Glasgow to Castle Douglas. The 2004 Tour of Britain was watched by hundreds of thousands of spectators, and this year's is expected to be even more popular.
Sports minister Richard Caborn said: "I was delighted to start the third stage of last year's Tour of Britain and it was obvious how much real enthusiasm there was for the event.
"I am confident that we will see more and more people taking up cycling - some to keep fit, some hopefully to produce the next generation of Olympic champions and Tour winners."