Mark Foster's bid to make the British team for the Olympics has ended.
Foster was not included in the squad after failing to make the 50m freestyle qualifying mark at the trials.
The 34-year-old appealed on the grounds that a shoulder injury had hampered him but an independent panel unanimously rejected his case.
British Swimming said it would be "a massive disappointment" to Foster but it hoped he would continue to represent Great Britain in future events.
Foster, the world silver medallist, missed the qualifying mark at the British trials by 0.05 seconds.
After he was excluded from the British team, he headed to the European Championships in an attempt to win a wildcard for the Games.
However, he finished fifth in the final, and was 0.15secs outside the qualifying mark.
Foster criticised his non-selection, saying: "I can't believe the criteria do not include provision for wildcard selection. I could win a medal for Great Britain."
National performance director Bill Sweetenham commented: "I feel sorry for Mark. It looks like the end of a career.
"But the rules are the same for everybody and there has to be a cut-off somewhere. He is the most experienced swimmer in Britain and he misjudged.
"The Olympic environment is unforgiving. To prepare for that, we have to emulate some of those conditions at the trials."
The British selection policy stated swimmers had to finish their respective final at the British Olympic Trials in first or second place while recording a time equal to, or faster than, the 12th fastest time in the world last year.