Henry has now amassed two golds and two world records in Athens
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Australian swimmer Jodie Henry set a new world record in qualifying for the women's 100m freestyle final.
Henry, who already has a gold from the 4x100m freestyle relay, put in a burst of speed in the last 25m to win her semi-final in 53.52 seconds.
But her relay team-mate Lis Lenton, who held the previous mark of 53.66 secs, surprisingly failed to qualify.
The other big names, defending champion Inge de Bruijn and American Natalie Coughlin, made it safely through.
Lenton finished fifth in her semi-final and her time of 55.17 secs was the ninth-fastest of the two semis - with only the first eight going through.
It was Henry's second world record of the meeting after the Australians set a new best time in the women's 4x100m freestyle relay.