Leontien Zijlaard-Van Moorsel became the most successful woman in Olympic cycling history after she powered to the time trial title on day five.
Van Moorsel added another gold to her three from Sydney
The Dutch rider gritted her teeth after suffering a nasty fall in the women's road race to complete the 24km-course in 31 minutes, 11.53 seconds.
The 34-year-old is the first female cyclist to win four Olympic gold medals on the road or in the velodrome.
She is bidding for a fifth gold in the track individual pursuit on Saturday.
Zijlarrd-van Moorsel thought her Olympics were over when she took a tumble after clipping the rider in front of her in the road race.
"After such a crash, I could never have dreamt something like that," she said after successfully defending her time trial title.
"I have pain everywhere and it was getting worse through the race. I don't think
elite sport is that healthy."
The Dutchwoman is retiring after the Athens Games but her tally of four golds and a silver saw her eclipse athletics great Fanny Blankers-Koen as the Netherlands most successful female Olympian.
American Deirdre Demet-Barry took the silver medal 24.09 seconds behind Zijlarrd-van Moorsel while Karin Thuerig of Switzerland took bronze.