World record holder Tim Montgomery will not run in the 100m at the Olympics after coming seventh in the US trials.
Reigning Olympic champion Maurice Greene won the race, ahead of Justin Gatlin and Shawn Crawford.
Montgomery's failure came a day after girlfriend Marion Jones also failed to qualify in the women's 100m.
Montgomery is fighting a possible lifetime ban on doping charges, while Jones is under investigation by the United States Doping Agency.
"This is the reason I didn't win," Montgomery said to reporters after his disappointing finish.
"I got you all on my back every day."
Montgomery is one of four sprinters charged by USADA with steroid use, even though he has never given a positive test, and is awaiting a hearing at the Court of Arbitration for Sport.
Of the others, Chryste Gaines failed to qualify in the women's 100m while Alvin Harrison made it through the first round of the 400m on Sunday.
And former world indoor 200m champion Michelle Collins withdrew from the women's 400m qualifying event with a leg injury.
Jones, who is still hoping to make the Olympic team for the 200m and long jump, has not been charged with any doping offence.
Greene clocked 9.91 seconds to win a high quality final, and immediately set his sights on retaining his Olympic crown.
"One down, one to go," he said.
"I have to go and win in Athens now. That's my goal.
"It's all about the G.O.A.T." he added, referring to the acronym tattooed on his arm which stands for Greatest Of All Time.