Rossi has been ahead of the field in both testing sessions so far
Valentino Rossi kept up his impressive pre-season form as he smashed his own pole-position record at the second test of the year at Sepang in Malaysia.
Having been fastest both days of the first test the reigning MotoGP world champion was again fastest on his Yamaha on both days of testing.
His time was two minutes 00.271 seconds with Casey Stoner second in 2:00.512.
Stoner's Ducati team-mate Nicky Hayden was third, with fellow Americans Colin Edwards and Ben Spies fourth and fifth.
Rossi's Fiat Yamaha team-mate Jorge Lorenzo, who was third fastest at the first testing session, was missing because of a hand injury.
It is still unclear if the Spaniard will be fit for the third and final testing session at Qatar on 18-19 March, only a matter of weeks before the 2010 championship starts at the same venue on Sunday 11 April.
Lorenzo finished runner-up to rival Rossi in last year's championship, and if he were to complete only one test in the run-up to this year's championship, it would be a huge setback.
The pair have confirmed they will continue to have a wall down the middle of their garage in 2010, a physical sign of the intensity of their rivalry.
Lorenzo even spoke seriously about leaving the team last year, saying he did not always feel appreciated, before signing a new one-year deal.
Rossi has hit back by making it clear he does not feel there is room for both riders in the team.
Malaysia MotoGP pre-season testing times:
1 V Rossi (Ita) Fiat Yamaha 2:00.271
2 C Stoner (Aus) Ducati 2:00.512
3 N Hayden (USA) Ducati 2:00.703
4 C Edwards (USA) Monster Yamaha Tech 3 2:00.988
5 B Spies (USA) Monster Yamaha Tech 3 2:01.014
6 L Capirossi (Ita) Rizla Suzuki 2:01.320
7 A Dovizioso (Ita) Repsol Honda 2:01.439
8 D Pedrosa (Sp) Repsol Honda 2:01.478
9 H Aoyama (Jpn) Interwetten-Honda MotoGP 2:01.692
10 A Bautista (Sp) Rizla Suzuki 2:01.732
11 R de Puniet (Fr) LCR Honda 2:01.980
12 M Melandri (Ita) San Carlo Honda Gresini 2:02.078
13 H Barbera (Sp) Aspar Team 2:02.080
14 M Kallio (Fin) Pramac Green Team 2:02.082
15 A Espargaro (Sp) Pramac Green Team 2:02.367
16 M Simoncelli (Ita) San Carlo Honda Gresini 2:03.689