Wilson held off the challenge of Nick Dougherty for a Ryder Cup spot
Oliver Wilson, Justin Rose and Soren Hansen booked the last three automatic spots for the European Ryder Cup team at the Johnnie Walker Championship.
Wilson, in the 10th and last qualifying place at the start of the week, held off a spirited challenge from Nick Dougherty to book a Ryder Cup debut.
Rose and Hansen qualified in eighth and ninth, before captain Nick Faldo added wildcards Ian Poulter and Paul Casey.
France's Gregory Havret (-14) won by one from England's Graeme Storm.
Another Englishman David Howell and Sweden's Peter Hanson tied for third on 11 under.
Colin Montgomerie failed to make an impact on the last day.
The Scot, an eight-time Ryder Cup veteran, needed a wildcard to make the European team but ended on one over at Gleneagles.
"If I get selected it'll be fantastic. If I don't I wish the team every success," said the 45-year-old Scot.
Wilson, 27, will be the first European to play in the Ryder Cup without first winning on tour.
"If I was a wildcard then I'd possibly feel like I don't really deserve to be there," Wilson said.
"But doing it without winning shows I've played pretty consistent. I think it is maybe a bit more impressive."
Wilson, who has had four second places this season, including at the prestigious PGA Championship at Wentworth, said his successful US college career should stand him in good stead in the Ryder Cup in Kentucky.
"There are probably 20 to 25 guys over there I've played with, I've beaten them and I know their games," he said.
"I played with Phil Mickelson this year and beat him at Loch Lomond, so it's not likely I'll be intimidated."
The Mansfield star carded a final-round 69 to end tied 10th on seven under, but crucially, Dougherty needed to finish second to have any chance of passing his fellow Englishman in the standings.
Despite beginning with three bogeys in his first six holes, Dougherty fought back with a 70 to end tied seventh on eight under.
"I'm proud of what I've achieved this week and my Mum would have been proud," said Dougherty, whose mother died for months ago.
England's Rose, 28, who will also be making his debut at Valhalla, ended tied fifth at 10 under after a closing 71 at Gleneagles, while Denmark's Hansen, 34, also booked his first appearance in the biennial event despite a disappointing 75 to finish seven under.
"Making it is the highlight of my season, but I want to be a valuable member of the team," said Rose.
Hansen added: "I'm very, very pleased - it's going to be a thrill."
Europe, the defending champions, face the United States on 19 September at Valhalla Golf Club in Kentucky.
The top five players from a list based on the points accrued from world rankings plus the next five best players on a European Tour-based points list make up the 10 automatic qualifiers.