Masters champion Tiger Woods' record run of 142 successive cuts came to an abrupt end at the Byron Nelson Championship in Irving, Texas.
Woods finished with a two-over par round of 72 in windy conditions
The world number one followed his opening one-under 69 with a two-over 72 in the second round to finish one over par on 141.
"I just didn't quite have it," said Woods after missing the level-par cut.
Woods was level with one hole left but bunkered his approach at the 18th and missed his 15-foot par-saving putt.
The last time he failed to make the cut was the 1998 Pebble Beach National Pro-Am, where he withdrew from the event.
The 29-year-old has only missed the cut twice in his career - the other time being at the 1997 Canadian Open, where he also bogeyed the last hole.
LONGEST CAREER CUTS ON US TOUR
Tiger Woods 142
Byron Nelson 113
Jack Nicklaus 105
Hale Irwin 86
Dow Finsterwald 72
Tom Kite, Vijay Singh 53
Woods now plans to spend the weekend out on the practice range at his home in Florida.
And the American did his best to shrug off letting his record slip away in windy conditions.
"What is it? Seven years? That's not too bad," Woods said.
"You've got to have some good breaks along the way and I've definitely had my share, but also I've gutted it out at times where, like today, I didn't feel very good.
"I just tried to bandage my way to the finish. I figured it was even par and I needed to make par.
"(The worst thing is) I don't get that opportunity to win the tournament."
Woods' run came to an end not only on a fitting day - Friday the 13th - but also at an apt event.
Byron Nelson held the previous record for the most consecutive cuts, 113, in the 1940s. Woods broke that record in the 2003 Tour championship.
However, Woods' record cut run did include 31 events without a cut, so technically his record stood at 111 cuts in a row.
World number three Ernie Els now has the longest active cut run at 20 tournaments.
Ten shots better off than Woods at the Byron Nelson Championship are joint leaders Sean O'Hair and Brett Wetterich.
The American duo have a one-shot lead over compatriot Ted Purdy.
World number two Vijay Singh is four shots off the lead alongside world number four Phil Mickelson. Ernie Els, ranked third in the world, is a shot further back.