Santos has scored 18 goals in 28 games for Tunisia
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Tunisia striker Francileudo Santos has been ruled out of the North African side's World Cup opener against Saudi Arabia on 14 June with a shin injury.
Santos has had the injury for the last two weeks and he aggravated it in a friendly against a German amateur side.
"We know he's going to miss the first game for sure, and after that we don't know," said midfielder Mehdi Nafti.
Brazilian-born Santos has scored 18 goals in 28 games since taking Tunisian nationality in 2003.