Midfielder Deco will miss Portugal's quarter-final with England after Fifa dashed any hopes of appealing against his dismissal in the game with Holland.
Deco is shown his second yellow card by referee Ivanov
Coach Luiz Felipe Scolari asked Portugal's Football Federation to appeal against Deco's first caution.
But Fifa communications director Markus Siegler said: "There was a phone call, they were clearly told 'forget it'."
Deco, one of four players sent off in the stormy second round tie, will serve a one-match ban.
The Barcelona midfielder was shown two yellow cards by referee Valentin Ivanov - the first for a foul on Dutch defender Johnny Heitinga and the second for delaying the game.
Portugal's midfield will also be missing Costinha, who was one of a World Cup record-breaking four players dismissed in the match.
Scolari accepted the two yellow cards shown to Costinha, the first for a tackle on Philip Cocu and the second for deliberate handball.
But Luis Figo will be able to face England after Fifa decided to take no further action on the yellow card shown to him for headbutting Mark van Bommel.