Neville has made 80 caps for his country
England defender Gary Neville has suffered a setback in his hopes of returning to full fitness at the World Cup finals in Germany.
The 31-year-old was hoping to return from a calf problem for the quarter-finals, providing England progress past Ecuador.
On Saturday, England coach Sven-Goran Eriksson said: "He worked yesterday out on the pitch but felt it this morning."
Eriksson added: "So it is one step backwards for him unfortunately."
After a scan on Wednesday, Neville had shown significant improvement but he now looks to be a major doubt for the tournament.
Neville took up his usual right-back position for England's opening 1-0 victory over Paraguay on 10 June.
However, the Manchester United player picked up the problem during training and sat out England's final group games against Trinidad and Tobago and Sweden.
Liverpool's Jamie Carragher has slotted in for Neville during those matches but Owen Hargreaves looks set to take his place for the second round game against Ecuador on Sunday.