Ecuador coach Luis Fernando Suarez says his side are determined to win Group A after their historic qualification.
A 3-0 win over Costa Rica sealed their place in round two for the first time, and a draw with Germany on Tuesday will see Ecuador top the group.
"Today is a very important day for Ecuador, a historic day," said Suarez.
"Our idea is to finish as the best team in the first round and then do the same in the second round and continue to improve as much as possible."
Despite a seven-hour time difference, fans in the Ecuador capital Quito poured out on to the streets after the match.
They blew car horns and jubilantly waved the country's yellow, red and blue flags.
"It's a day of celebration," said Colombian-born Suarez.
"We have never qualified for the second stage and this is a fantastic achievement for us."
We think about winning every game
Ecuador striker Agustin Delgado
The group winners will face the runners-up, rather than the top team, from England's Group B.
And Ecuador will finish top ahead of fellow qualifiers Germany with a draw in their third group match because of superior goal difference.
"We must now enjoy this moment and then concentrate on making a good performance against Germany before we find out who our next rival will be," said Suarez.
"We haven't come to the World Cup just for the ride.
"We want to have a starring role and we now want more."
Agustin Delgado and Ivan Kaviedes celebrate the win
Strikers Carlos Tenorio and Agustin Delgado both scored their second goals of the tournament in the win over Costa Rica.
Tenorio said: "We have not achieved anything yet, we want to go as far as we can."
Delgado added: "We're very happy for having qualified, aside from me having scored another goal for my team.
"We're now thinking about being group leaders. We think about winning every game."