Argentina coach Jose Pekerman has left former captain Javier Zanetti out of his World Cup squad.
Zanetti has been left out of Argentina's squad
Bayern Munich midfielder Martin Demichelis also missed out but uncapped Independiente goalkeeper Oscar Ustari has beaten German Lux to a place.
West Ham midfielder Lionel Scaloni and Manchester United's fit-again defender Gabriel Heinze had already been told they were in the squad.
Barcelona forward Lionel Messi is included despite his calf injury.
Messi is one of several in the squad who starred for the Argentinian junior side that won the under-20 World Cup last year.
"There is a permament evolution with footballers. Several of the under-20 footballers have made themselves noticed," said Pekerman.
"Before it would have been impossible for players of this category to come into the A team."
Boca Juniors striker Rodrigo Palacio is rewarded for his excellent season with a place, as is fellow striker Julio Cruz.
Cruz scored 17 goals for Inter in Serie A and will provide an alternative to Hernan Crespo as a big target man up front.
His Inter team-mate Zanetti was left disappointed and said: "Nobody told me what I did wrong to not be in the World Cup squad.
"I'm calm because I think I did my best to be on the list and I've been always available for the national team when they needed me.
"We'll never know if I've been excluded because of football reasons or not."
Argentina play their opening Group C match against Ivory Coast on 10 June 10 and will also meet Serbia & Montenegro and Holland.
Goalkeepers: Roberto Abbondanzieri (Boca Juniors), Leonardo Franco (Atletico Madrid, Spain), Oscar Ustari (Independiente)
Defenders: Fabricio Coloccini (Deportivo Coruna, Spain), Roberto Ayala (Valencia, Spain), Gabriel Heinze (Manchester United, England), Juan Pablo Sorin (Villarreal, Spain), Gabriel Milito (Real Zaragoza, Spain), Nicolas Burdisso (Inter Milan, Italy), Leandro Cufre (AS Roma, Italy)
Midfielders: Lionel Scaloni (West Ham United, England), Esteban Cambiasso (Inter Milan, Italy), Javier Mascherano (Corinthians, Brazil), Luis Gonzalez (Porto, Portugal), Pablo Aimar (Valencia, Spain), Juan Roman Riquelme (Villarreal, Spain), Maximiliano Rodriguez (Atletico Madrid, Spain)
Forwards: Carlos Tevez (Corinthians, Brazil), Hernan Crespo (Chelsea, England), Lionel Messi (Barcelona, Spain), Rodrigo Palacio (Boca Juniors), Javier Saviola (Sevilla, Spain), Julio Cruz (Inter Milan, Italy)