Wrexham defender Shaun Pejic will be out for around two months after tearing cruciate knee ligaments in Tuesday's 1-0 Carling Cup defeat to Doncaster.
It was initially feared he would miss six months, but a scan revealed he is unlikely to need an operation.
The 22-year-old suffered the injury in a challenge with Ricky Ravenhill, who was immediately taken off by Rovers.
"We'll see on the video, but I'm pretty sure it wasn't the best of challenges," said Wrexham boss Denis Smith.
Dean Bennett and Dennis Lawrence are also doubtful for Saturday's League match after picking up injuries.
Lawrence pulled a hamstring in the warm-up, while Bennett came off with a groin problem in the closing stages.
Matt Crowell, Lee McEvilly, Lee Roche and Jim Whitley were already out injured, with Simon Spender suspended.
"I'm not sure who's run over the black cat, but somebody's done something because this is absolutely ridiculous," said Smith after the game, when he felt Pejic would be out for a longer period.
"For Dennis Lawrence to break down during the warm-up... he never misses a game.
"Shaun Pejic is the major blow. You can talk about going out of the Cup but we've lost a very important player who's been having a superb season."
He added: "I don't think we deserved to get beat but in the end we've got to stick the ball in the net and keep it out at.
"But considering what had gone on, the fact that we had to change everything 10 minutes before kick-off, I mean Andy Hole didn't have a clue he was going to be playing centre back until five minutes before he went out.
"So it's a good performance against a team from a higher division.
"But we got beat 1-0 again and that's what people will see."
Smith said he would be looking to bring in players on loan to ease the injury crisis - providing he gets permission from the Football League.
Wrexham, who have gone four games without a goal, are still constrained by the League's rules governing clubs in administration.