Wolves are ready to take a tough stance if striker Henri Camara fails to return to Molineux for pre-season training.
Senegal striker Camara has boycotted training in protest at the club's rejection of Bolton's £2.5m offer.
Wolves chief executive Jez Moxey said: "He has a contractual and moral responsibility to help us regain our Premiership status.
"We're making a stand for what we believe is right and what we believe is for the good of football."
Wolves issued a hard-hitting statement on their website, hinting that Camara had been unsettled by Bolton's interest.
Moxey said: "We know he has been influenced by the interest of Bolton Wanderers.
"It may or not be a coincidence but Bolton faxed their one-and-only offer of £2.5m one hour prior to Henri and his advisors arriving at Molineux for a meeting on Monday.
"That was an offer Phil Gartside, the Bolton chairman, said he would not make but asked me to keep him informed if we decided to sell Henri in the future - a commitment I gave to him in good faith.
"The player has been unsettled and we are unhappy about what appears to have been going on behind the scenes."
Moxey confirmed that Wolves are determined to take a hard line with Camara - if he fails to show up for pre-season training.
He added: "It is very disappointing that Henri failed to participate in pre-season training - as instructed in a meeting with me - and has subsequently failed to report along with the rest of the players.
"The player and his advisors are wrong in what they are doing and they must understand that their actions have consequences.
"We want Henri to return to pre-season training immediately.
"If he doesn't then ensuing disciplinary procedures will involve ongoing fines.
"Henri wants to play in the Premiership but he has a moral and contractual responsibility to do his best to help Wolves regain the Premiership status, which was lost last season with him as a regular team member.
"We feel he owes that to the club, the manager, his team-mates and Wolves supporters everywhere."
Wolves had described Bolton's offer as "unacceptable" and Moxey insisted they would not be held to ransom by Camara.
He added: "Some clubs would be forced to accept the apparent commercial reality of this situation - that it is better to cash in and make a profit on a player who wants to be transferred to a Premiership club rather than run the risk of devaluing your asset by keeping an unhappy player.
"Well, we want Henri to try his best to help the team win promotion at the first attempt and we will not, therefore, be forced to sell him to another club unless it is on terms acceptable to us."