Weymouth have placed all of their first-team squad on the transfer-list following their decision to axe manager Garry Hill and assistant Kevin Hales.
Hill was sacked by the club after guiding them to promotion
The moves are designed to cut costs at the club, which would not have been able to sustain its current level of spending on its playing budget.
The Conference side's chairman, Martyn Harrison said: "We have no other option than to reduce our costs.
"This is painful but we're confident we have made the right decisions."
He added: "It is important to stress at this point that this club has not gone bust, is not going bust and continues to have my fullest support.
"We will continue to meet our commitments to play all the fixtures planned between now and the end of this season.
"I cannot emphasise enough that we are making these changes in order to guarantee the future of this club."
Chief executive Gary Calder has been placed in charge of the day-to-day running of the club, including the recruitment of players.
They are likely to be needed should the majority of Weymouth's current squad find alternative clubs during the transfer window.
Captain Ben Smith has already been targeted by Bournemouth boss Kevin Bond.
Weymouth are currently seventh in the Conference top-flight after winning the Conference South title last season but any hopes of pushing for promotion to the Football League appear to have ended.