Tottenham skipper Robbie Keane has had his claim for wrongful dismissal rejected by the Football Association.
Keane set up one and scored two against Bolton before being sent off
Keane was sent off for denying a scoring opportunity 36 minutes into the weekend win against Bolton when referee Graham Poll deemed he handled the ball.
Wanderers scored from the resulting spot-kick but failed to rally against 10 men, finally losing the match 4-1.
The Irish striker must now serve a one-match ban, meaning he will miss Sunday's tie at London rivals West Ham.
Keane scored a brace before being sent off for the first time in his career and was furious with the decision, which came after he blocked a goalbound Ivan Campo header on the line.
The ball struck his chest before appearing to hit his arm as it dropped and Poll issued a straight red card for denying a goalscoring opportunity.
"Robbie said that he had chested the ball and it was definitely not a penalty," said Spurs boss Martin Jol after the match.
"It's always easy in hindsight. If you win games it's not a problem. He said he chested it."