Scarborough manager Nicky Henry has left the Conference strugglers after Saturday's FA Cup defeat at Harrogate.
Neil Redfearn has been named as caretaker manager for Saturday's game with Aldershot Town.
"It's a difficult time for everyone associated with the club. I'm asking the fans to continue supporting the club," said chairman Malcolm Reynolds.
"The 700 who travelled to Harrogate were a credit to the club and the team will need that support on Saturday."
Henry, 36, joined Scarborough as a player in 2000 and took over as manager in May 2004.
Scarborough are bottom of the Conference with three wins this season.