Raith Rovers assistant boss John Hollins has parted company with the club after only a month in charge.
Claude Anelka has already split with former assistant David Martin
The ex-Chelsea manager is thought to have clashed with manager Claude Anelka over team selection since his arrival at Stark's Park.
Anelka bought the First Division club in the summer, but they have slumped to bottom under his leadership.
"I have enjoyed my month here and wish the players, the board and the club all the best," said Hollins.
Angry Rovers fans are expected to host a demonstration against Anelka at Saturday's game with Queen of the South, with reserve coach Shaun Dennis taking over on a temporary basis.
Anelka, brother of Manchester City striker Nicolas, had no previous experience of coaching at senior level but took charge of the team in return for a £300,000 investment.
The 35-year-old has revamped the squad, but his team lie five points adrift at the bottom, having won just one league point this season.
Former assistant and director of football David Martin also quit the Kirkcaldy club last month, while strikers' coach Gordon Wallace left for Dundee.
He last month brought in Hollins, the former Chelsea, Swansea and Rochdale boss who also managed Chinese club Stockport Tiger Star.
Previous reports have suggested that Anelka was contemplating reverting to a director of football role following disagreements over players and tactics.