Pompey last competed in Europe during the 2008/2009 season
Portsmouth will not be allowed to play in next season's Europa League after a joint decision by the Football Association and Premier League.
The cash-strapped club had appealed to the FA asking for a Uefa licence to compete in Europe - despite missing the deadline for applications.
Portsmouth would have qualified for the competition if they were to beat Chelsea in the FA Cup final on 15 May.
The team finishing seventh in the Premier League will now qualify.
Pompey, facing debts of £119m, were not in a position to file their accounts in time because the club is in administration - a criterion for a Uefa club licence.
A place in the Europa League is handed to the winners of the FA Cup, while two other Europa League places are handed to the fifth-placed team in the Premier League and the winners of the League Cup.
Yet with Manchester United already winning that trophy, a subsequent spot will be handed to the sixth-placed team in the league, while seventh place will also qualify.
It's wrong for the fans that they should not be allowed to support their club in Europe next season
Portsmouth administrator Andrew Andronikou
Uefa delegates its licensing scheme to national associations, leaving the FA to decide whether Portsmouth could prove to Uefa that the business was solvent by 31 May.
However, after deliberating with the Premier League, English football's governing body opted against endorsing Portsmouth's application.
"The FA and Premier League have confirmed to the administrators of Portsmouth Football Club that they shall not consider any late application for granting of a Uefa Club Licence for the 2010-11 season," read a joint statement.
The main issues were there should be no late payments outstanding to other clubs, or to staff and players.
Already relegated from the Premier League, Portsmouth administrator Andrew Andronikou believes the ruling is unfair on the club's long-suffering supporters.
"We have spent a long time going through all the necessary steps and we had hoped to make an application next week," he said.
"We felt that we would do our bit and that it would be up to the FA and the Premier League to do theirs.
This page is best viewed in an up-to-date web browser with style sheets (CSS) enabled. While you will be able to view the content of this page in your current browser, you will not be able to get the full visual experience. Please consider upgrading your browser software or enabling style sheets (CSS) if you are able to do so.